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Elevation of granulocyte-macrophage colony-stimulating factor (GM-CSF)-producing T helper (TH) cells has been associated with several autoimmune diseases, suggesting a potential role in the pathogenesis of autoimmunity. However, the identity of GM-CSF-producing TH cells has not been closely examined. Using single-cell RNA sequencing and high-dimensional single-cell mass cytometry, we identified eight populations of antigen-experienced CD45RA-CD4+ T cells in blood of healthy individuals including a population of GM-CSF-producing cells, kno
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