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By analyzing real-time trajectories of fluorescent antibodies against a virus surface necessary protein with regards to single virus-derived Rayleigh scattering, we determined heterogeneous binding stoichiometry of virus-antibody couplings irrespective of the nonspecific binder population. Therefore, our multimodal (or multi-level) SMSP assay visually identifies and selectively quantifies certain communications among them with solitary binding occasion accuracy. As a 'specific-binding
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