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The most favorable pulse shape is either ramp-up or triangular. Isolation precautions are recommended when caring for patients identified with highly resistant micro-organisms (HRMOs). However, the direct costs of patients in isolation are largely unknown. To obtain detailed information on the daily direct costs associated with isolating patients identified with HRMOs. This study was performed from November until December 2017 on a 12-bed surgical ward. This ward contained solely isolation rooms with anterooms. The daily direct costs of