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09 mPa. At 10 mPa, both upregulation of osteogenic genes and higher alkaline phosphatase activity were observed. These results suggest that the intermittent shear of the order of 10 mPa can competently enhance osteogenic differentiation of rBMSCs compared to static culture.Observations and analyses of a microscopic object are essential processes in various fields such as chemical engineering and life science. Microfluidic techniques with various functions and extensions have often been used for such purposes to investigate the mechanical p
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