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It may improve the microbial activity of the soil, and that could be a new technique to stimulate earth virility without producing any unfavorable impact on crop production.The effect of subacute rumen acidosis (SARA) from the rumen microbial neighborhood has been frequently studied in in vivo studies. Here we investigated whether these modifications can be mirrored by using the rumen simulation method (RUSITEC) as an in vitro model for this condition. We hypothezised that the microbia