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C99 and the production of Aβ.Molecular assembly is crucial in functional molecular materials and devices. Among the molecular interactions that can form assemblies, stacking among π-conjugated molecular backbones plays an essential role in charge transport through organic materials and devices. The single-molecule junction technique allows for the application of an electric field of approximately 108 V/m to the nanoscale junctions and to investigate the electric field-induced assembly at the single-stacking level. Here, we demonstrate