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OBJECTIVE This study aims to determine estradiol levels in mothers who experience postpartum blues. METHODS It was a crossectional study conducted in Pertiwi Maternal and Child Hospital Makassar. The population was all mothers who had normal postpartum births on the third day from January to March 2018, and samples were 31 selected accidentally selected. Postpartum blues data was obtained with the Edinburgh Postnatal Depression Scale (EPDS) questionnaire and secondary data obtained from maternal medical records in the delivery room and nursing staff at the