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Chlorophytum comosum popularly known as Spider Ivy is used as medicinal plant in traditional Chinese medicine, however its detailed chemical composition and biological activity is yet unexplored. To carry out phytochemical investigation on different parts of Chlorophytum comosum using GC-MS/LC-ESI-MS and evaluation of its antioxidant, haemolytic and antiproliferative potential on breast cancer (MCF-7), lung cancer (A549, H1299) and normal lung (L-132) cell lines. Chemical constituents from aqueous roots and leaves extracts were identif