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5%) with IC50 = 0.2% for both of the cell lines. Eucalyptus essential oil showed some side effects and might not be safe to use for treatment at high doses (0.5% and 5%). Moreover, the findings of the present study show that eucalyptus essential oil suppressed the proliferation of human colon cancer cells by inducing apoptosis. Eucalyptus essential oil might be a good candidate for the treatment of colon cancer. Eucalyptus essential oil showed some side effects and might not be safe to use for treatment at high doses (0.5% and 5%). More