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To facilitate the simultaneous determination regarding the neurotransmitters, a chemometric tool of Tchebichef bend moment (TcM) technique had been suggested. The TcMs calculated through the voltammograms were used to determine the quantitative designs by stepwise regression. The intra-day and inter-day precisions of this proposed strategy had been lower than 3.5% and 8.1%, respectively, in addition to recoveries were from 87.4% to 124%. The limitation of recognition (LO