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Incubation of this immunoprecipitates with all the overabundance the contending peptide allows the elution associated with captured proteins without contamination associated with test with all the antibodies present in the immunoprecipitates. But, this method just isn't constantly offered, as well as the cost of contending peptide may be prohibitive for the routine immunoprecipitation/immunoblotting experiments. In this protocol, elution regarding the immunoprecipitated proteins from the beads is carried ou