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The CD138int FO B cells revealed an anergic gene phrase profile shared with compared to monoclonal anergic B cells expressing engineered BCRs and also the gene appearance profile ended up being not the same as those of plasma cells, age-associated B cells, or germinal center B cells. The anergic state of the CD138int FO B cells was confirmed by attenuated Ca2+ reaction and failure to upregulate CD69 upon BCR engagement with anti-IgM, anti-IgD, anti-Igκ, or anti-I