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We produced sequence alignments for each family using two well-known sequence aligners (MUSCLE and MAFFT). We found KnotAli's performance to be superior in 6 of the 10 families for MUSCLE and 7 of the 10 for MAFFT. While both KnotAli and Cacofold use background noise correction strategies, we found KnotAli's predictions to be less dependent on the alignment quality. KnotAli can be found online at the Zenodo image https//doi.org/10.5281/zenodo.5794719. We found KnotAli's performance to be superior in 6 of the 10 families for MUSCLE and 7