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Additionally, both 2D and 3D imaging provides a means to acquire and analyze entirety of the sample. © 2020 The Authors. Published by Elsevier B.V.The total phosphorus analyte (TP) has a long history of use in monitoring and regulatory applications relating to management of cultural eutrophication in freshwaters. It has become apparent, however, that the fraction of the TP analyte ultimately available to support algal growth varies significantly spatially (within a system), seasonally, and among systems. The algal bioassay methods descri