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However, also this design offered limitations. One becoming its opaqueness managed to get hard to image the undamaged tissue. Another draw-back was that tumor cells upon intrusion utilized the scaffold as a guardrail abandoning an unspecific intrusion structure. All this might be avoided by a method, the fibroblast-derived matrix-based model, on the basis of the work by Ahlfors and Billiar (2007) We here provide a protocol with this variety of model, therefore supply