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All in all, our findings indicate differential nano-specific toxicity compared to ionic Cu2+ toxicity in soybean. These results may guide researchers and regulators on how best to tailor ENMs with specific particle characteristics rendering them more or less toxic, and better inform risk assessment of CuONPs in soil grown food crops such as soybean. With increasing population, rapid urbanization, and increased migration to cities, the local impacts of increasing transportation and industrial-related air pollution are of growing concern w