https://www.selleckchem.com/pr....oducts/way-100635.ht
To study the expression of Shh singaling related gene, including Shh, Ptch1, Smo and Gli1 in bone marrow CD34 cells of patients with myelodysplastic syndrome(MDS) and acute myeloid leukemia with myelodysplasia-related changes(AML-MRC), and to explore their clinical significance. The count of CD34 cells in bone marrow was detected by flow cytometry in 53 patients with MDS and 30 patients with AML-MRC. Magnetic beads were used to separate CD34 cells. The expression of Shh, Ptch1,Smo and Gli1 on CD34 cells was detected by qRT-PCR, the e
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