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Finally, the product with more nC -surfactin isoform had a higher hydrocarbon-emulsification index, and it increased the water-wettability of the oil-saturated silicate surface. The obtained results identified that enhancing the supply of fatty acid precursor is very essential for the synthesis of surfactin. At the same time, this study also proved that thioesterase BTE can promote the production of nC -surfactin and experimentally demonstrated its higher surface activity and better performance in oil-washing. These results are of great s