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The work aimed to enhance the extraction of wheat germ polysaccharides (WGPs) by hydrothermal treatment and identify the structural properties of WGPs. Results showed that the extraction yield of crude WGPs reached 18.72% after steam explosion. A homogeneous polysaccharide fraction (steam explosion wheat germ polysaccharides, SEWGPs) was also released and purified by DEAE-cellulose-52 separation and Sephacryl S-500 HR gel filtration with a yield of 3.84%. The SEWGPs had an average molecular weight of 3.34 × 106 Da and was composed of g