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cell; TCF-1 transcription factor T-cell factor 1; TOX Thymocyte selection-associated high mobility group box protein; NFAT nuclear factor of activated T cells; NA Naïve; CM Central Memory; EM Effector Memory; EMRA Terminal Effector Memory cells; FMO Fluorescence minus one; PVR poliovirus receptor; PVRL2 poliovirus receptor-related 2; IFN-γ Interferon-γ; IL-2 interleukin-2; MCF multiparametric flow cytometry; TNFα Tumornekrosefaktor α; RT room temperature. Tibiotalocalcaneal (TTC) intramedullary nailing has been suggested as an alternati