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1 mV) after a train of ten stimuli were applied at 5 Hz, and deltamethrin at 0.03 μm showed significant membrane depolarization of 2.9 ± 0.4 mV without stimuli. 2S at 0.3 μm and 4-AP at 1-3 μm significantly synergized the effects of 3 μm permethrin and 0.01 μm deltamethrin. Co-application of potassium channel blockers 2S and 4-AP with pyrethroids can synergize the mosquitocidal activities on An. gambiae, and these activities are correlated with synergistic effects at the level of the nerve membrane. If deployed in the field, this appro