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In our research we established a recombinant expression system for barley magnesium chelatase with all the long-term objective to acquire structural information of this enigmatic chemical complex from an increased plant. The genetics Xantha-h, -g and -f were cloned in plasmid pET15b, which permitted the production associated with three subunits as His-tagged proteins in Escherichia coli BL21(DE3)pLysS. The purified subunits stimulated magnesium chelatase task of barley plastid extracts and created activ
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