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The phrase of six biofilm-related genes-biofilm peroxide weight protein (bsmA), biofilm formation regulatory protein subunit R (bssR), biofilm formation regulatory protein subunit S (bssS), biofilm development regulator (hmsP), toxin-antitoxin biofilm protein (tabA) and transcriptional activator of curli operon (csgD)-and two virulence factors-Vi antigen-related gene (via and Shiga-like toxin (slt-II)-was examined by RT-PCR. A. hydrophila displayed a 2-fold increase in slt-II expression in