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84 Hz (0.52 Hz), amplitude of 0.32 degrees (0.11 degrees), peak velocity of 43.68 degrees/s (14.02 degrees/s), and peak acceleration of 13,920.04 degrees/s2 (4,186.84 degrees/s2). The first trace recorded within a session significantly differed from the second two in both microsaccade acceleration and velocity (P 0.05), and frequency was 0.098 Hz higher in the evenings (P 0.05). There was no MM difference between days and no evidence of a session-level learning effect (P 0.05). The TSLO is both accurate and precise. Howeve