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uthor(s) 2020. Published by Oxford University Press on behalf of International Society for Diseases of the Esophagus. All rights reserved. For permissions, please e-mail [email protected] of Zika virus (ZIKV) RNA in semen is common after infection. We designed a RT-PCR assay that targets antisense ZIKV RNA (asRNA) to assess ZIKV replication competence in ZIKV RNA positive semen samples. We detected ZIKV asRNA in semen of nine of nineteen men (47.4%) diagnosed with ZIKV infection. All asRNA positive samples had hi