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By contrasting various serotypes of recombinant adeno-associated virus (rAAVs), we found out that rAAVs provide sufficient effectiveness for gene transfer with various tissue preferences according to the serotypes for the virus. AAV-2 and AAV-8 preferentially target epithelial and mesenchymal compartments, respectively, while AAV-9 infects both tissues. Right here, we describe a protocol for AAV-mediated gene transfer in ex vivo cultured murine embryonic mammary gland facilitat