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In comparison, the HYD2 homoeologs functioned toward production of lutein, the main carotenoid in mature grains, whereas HYD1 homoeologs had a limited part. These outcomes collectively advised subfunctionalization of HYD genetics and homoeologs in various cells of tetraploid wheat. Despite decreased photoprotective responses noticed in the triple hyd-A1 hyd-B1 hyd-A2 as well as the quadruple hyd-A1 hyd-B1 hyd-A2 hyd-B2 combinatorial mutants, comprehensive plant phenotyping