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We created a regular PCR and a quantitative PCR (qPCR) assay for the recognition of Pco considering its specific srlE gene encoding sorbitol-specific phosphotransferase. Those two practices could specifically amplify the anticipated services and products of 674 and 108 bp, respectively, from all of the Pco strains. The assays shown high sensitivity and might identify as low as 1 and 100 pg/µl of microbial genomic DNA, respectively. Both assays could also identify the pathogens directly fro