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Absolutely the configurations of compounds 2 and 3 had been dependant on Mosher's strategy and TDDFT-ECD calculations. All separated compounds had been evaluated for their cytotoxic and antimicrobial activities. Cladodionen (4) exhibited cytotoxicity against the mouse lymphoma cell line L5178Y with an IC50 value of 3.7 μM, and also exhibited antifungal activity against Ustilago maydis and Saccharomyces cerevisiae, while cladosin L (1) displayed week antibacterial task against Staphyloc