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Choosing the right microorganism, that could grow quickly on cheap feedstocks, is of crucial significance whenever building an economically feasible bioprocess. We selected Lactococcus lactis, a well-characterized lactic acid bacterium, as our microbial host to produce pyruvate, which can be a commodity substance with various essential applications. Here we report the manufacturing of Lactococcus lactis into getting an efficient microbial system for creating pyruvate. The strain obtained, FS