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Using HLA-A*0201- and HLA-DR*4-transgenic mouse designs, we discovered that DKK1-specific CD4+ and CD8+ T cell answers, detected by DKK1 short peptide (P20 and P66v)-HLA-A*0201 tetramer staining and cytotoxic assay for CD8+ T cells or by CSFE dilution and IFN-a; secretion for CD4+ T cells respectively, can be induced in vivo by immunizing mice utilizing the DKK13-76-LP. In addition, DKK13-76-LP also induced an