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001), but there was no significant decrease in group B (P=0.43). Biotin (10mg/day) given in addition to isotretinoin treatment decreased telogen and increased anagen hair rates and helped to maintain skin hydration. The use of 10mg/day biotin can prevent the mucocutaneous adverse effects of isotretinoin treatment. Biotin (10 mg/day) given in addition to isotretinoin treatment decreased telogen and increased anagen hair rates and helped to maintain skin hydration. The use of 10 mg/day biotin can prevent the mucocutaneous adverse effects