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It is believed that the developed SERS patch can be utilized as various flexible and wearable biosensors for healthcare monitoring.An effective and sensitive method for the determination of isopyrazam (IZM) isomers (syn-IZM and anti-IZM) and their metabolites (syn545364 and syn545449) in tomato and soil by ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed in the present study. The method showed excellent linearities (R2 = 0.999) at 0.005-5 mg/L. The recoveries were 92.0-107%, and the relative standard deviati