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MEPS aided by the after silylation resulted in the reproducible formation of types with an unsubstituted hydrogen ion in the indole ring, the chromatographic peaks of that are symmetric and may be used for GC-MS analysis without extra derivatization. The recoveries of analytes in the restriction of quantitation (LOQ) levels had been 40-80% for pooled CSF and 40-60% for serum. The limit of recognition (LOD) and LOQ values were 0.2-0.4 and 0.
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