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We develop a mathematical model to quantitatively describe the imbibition dynamics of an elastic non-Newtonian fluid in a conical (nonuniform cross section) microfluidic assay. We consider the simplified Phan-Thien-Tanner viscoelastic model to represent the rheology of the elastic non-Newtonian fluid. Our model accounts for the geometrical features of the fluidic assay, the key parameters affecting the rheological behavior of the fluid, and predicts the imbibition dynamics effectively. By demonstrating the temporal advancement of the fi