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AIMS Agro-based wastes were evaluated as a medium for mass micropropagule production and optimal efficacy of Trichoderma asperellum B1092 in controlling F. oxysporum f. sp. lycopersici and promoting tomato growth. This study focused on biological control because pathogen persistence in the soil makes the disease difficult to control. METHODS AND RESULTS Rice bran, biochar, empty fruit bunches, coconut fibers, compost, top soil and mixed soil were evaluated as media for mass multiplication of Trichoderma asperellum, which is effective in c