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Application of the method was undertaken by comparing urban and rural Xanthoria parietina samples to investigate the relative importance of the two nitrogen compounds in contrasting environments. Findings presented showed that lichen nitrogen compound concentrations varied in rural and urban X. parietina samples, suggesting different atmospheric nitrogen loadings from potentially different sources (e.g. agricultural and traffic) and varied deposition patterns (e.g. urban layout impacts). Despite potential impacts of nitrogen compounds on
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