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Graphical abstract.Setting of maximum limits for many plant alkaloids is under conversation in the EU. The novel strategy created and optimized in this study enables simultaneous determination of 21 tropane alkaloids (TAs) and 33 pyrrolizidine (PAs) as well as their particular N-oxides (PANOs). For analysis of aqueous-methanolic extract, reversed phase ultra-high-performance liquid chromatography and tandem mass spectrometry (RP-U-HPLC-MS/MS) ended up being employed. The strate
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