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The critical appraisal tool for prevalence studies designed by the Joanna Briggs Institute (JBI) was used to assess the risk of bias in all included studies. A total of 84 articles, covering 21 countries, were included in this review. Quantitative meta-analysis estimated a pooled IgG prevalence of 25% (95% CI 21-29%, I 2 = 99%), a pooled IgM prevalence of 10% (95% CI 9-11%, I 2 = 98%) and a pooled DENV RNA prevalence of 14% (95% CI 12-16%, I 2 = 99%). Evidence for possible publication bias was also found in all three meta-analyses. Subg