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Overall, our results indicated that damming induced changes in physiochemical variables (e.g., temperature, conductivity, and nutrients), accompanied by alterations in flow regime and longitudinal connectivity, increased replacement and loss of taxa or traits. These changes have consequently led to alteration of macroinvertebrate taxonomic and functional community dissimilarity and affected the relative effects of environmental and spatial factors on beta diversity and its components. Our study helps understand the ecological processes associated with dam i