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0005). Whereas, we did not observe any association between serum Klotho level with all-cause mortality and cancer-specific mortality (all p 0.05). Then, stratified analysis found that people aged 60-79, female, overweight and non-Hispanic whites or Mexican Americans were less likely to develop cancer. In addition, there was a strong nonlinear and linear positive correlation of Klotho with estradiol (p-nonlinear=0.0178) and testosterone only among male participants (β=-0.513, p=0.0137), respectively. We found an inverse association b